PrimePCR™ SYBR® Green Assay: CBR1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0035877

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CBR1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CBR1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0035052
Assay Design:   Exonic
Chromosome Location:   21:37442353-37442477question
Amplicon Length:   95
Splice Variants Targeted:   ENST00000530908 ENST00000290349 ENST00000439427 ENST00000399191

Gene Information

Carbonyl reductase is one of several monomeric NADPH-dependent oxidoreductases having wide specificity for carbonyl compounds. This enzyme is widely distributed in human tissues. Another carbonyl reductase gene CRB3 lies close to this gene on chromosome 21q. [provided by RefSeq Jul 2008]

Gene Symbol:   CBR1
Gene Name:   carbonyl reductase 1
Aliases:   CBR, SDR21C1, hCBR1
RefSeq:   NC_000021.8 NT_011512.11
Ensembl:   ENSG00000159228
Entrez:   873
Chromosome Mapping:   21q22.13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.970000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download