PrimePCR™ SYBR® Green Assay: SET, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041403

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SET, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SET, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038681
Assay Design:   exonic
Chromosome Location:   9:131456920-131457032question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000339554 ENST00000371482 ENST00000371475 ENST00000265663 ENST00000437259 ENST00000392812 ENST00000371474 ENST00000371473 ENST00000541195 ENST00000502935 ENST00000369680 ENST00000393583 ENST00000296440 ENST00000358536 ENST00000372692 ENST00000409104 ENST00000322030 ENST00000372686 ENST00000372688

Gene Information

The protein encoded by this gene inhibits acetylation of nucleosomes especially histone H4 by histone acetylases (HAT). This inhibition is most likely accomplished by masking histone lysines from being acetylated and the consequence is to silence HAT-dependent transcription. The encoded protein is part of a complex localized to the endoplasmic reticulum but is found in the nucleus and inhibits apoptosis following attack by cytotoxic T lymphocytes. This protein can also enhance DNA replication of the adenovirus genome. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Oct 2011]

Gene Symbol:   SET
Gene Name:   SET nuclear oncogene
Aliases:   2PP2A, I2PP2A, IGAAD, IPP2A2, PHAPII, TAF-I, TAF-IBETA
RefSeq:   NC_000009.11 NT_008470.19
Ensembl:   ENSG00000119335
Entrez:   6418
Chromosome Mapping:   9q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 37.780000
Efficiency 90

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download