PrimePCR™ SYBR® Green Assay: AGT, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0051890

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: AGT, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: AGT, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044628
Assay Design:   exonic
Chromosome Location:   1:230838316-230838416question
Amplicon Length:   71
Splice Variants Targeted:   ENST00000366667

Gene Information

The protein encoded by this gene pre-angiotensinogen or angiotensinogen precursor is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product angiotensin I is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia. Mutations in this gene are associated with susceptibility to essential hypertension and can cause renal tubular dysgenesis a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation and inflammatory bowel disease. [provided by RefSeq Jul 2008]

Gene Symbol:   AGT
Gene Name:   angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
Aliases:   ANHU, FLJ92595, FLJ97926, SERPINA8
RefSeq:   NC_000001.10 NG_008836.1 NT_167186.1
Ensembl:   ENSG00000135744
Entrez:   183
Chromosome Mapping:   1q42-q43

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.010000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download