PrimePCR™ SYBR® Green Assay: RAP1GDS1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052120

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RAP1GDS1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RAP1GDS1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044858
Assay Design:   exonic
Chromosome Location:   4:99182695-99214643question
Amplicon Length:   84
Splice Variants Targeted:   ENST00000509011 ENST00000380158 ENST00000264572 ENST00000508213 ENST00000408927 ENST00000514122 ENST00000453712 ENST00000511212 ENST00000408900 ENST00000339360

Gene Information

The smg GDP dissociation stimulator (smgGDS) protein is a stimulatory GDP/GTP exchange protein with GTPase activity (Riess et al. 1993 [PubMed 8262526]).[supplied by OMIM Feb 2010]

Gene Symbol:   RAP1GDS1
Gene Name:   RAP1, GTP-GDP dissociation stimulator 1
Aliases:   GDS1, MGC118859, MGC118861, SmgGDS
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000138698
Entrez:   5910
Chromosome Mapping:   4q23-q25

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 37.030000
Efficiency 91

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download