PrimePCR™ SYBR® Green Assay: TP53, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TP53, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TP53, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0013658
Assay Design:   Intron-spanning
Chromosome Location:   17:7577571-7578376question
Amplicon Length:   126
Splice Variants Targeted:   ENST00000269305 ENST00000455263 ENST00000420246 ENST00000445888 ENST00000509690 ENST00000514944 ENST00000413465 ENST00000359597 ENST00000396473 ENST00000419024 ENST00000545858

Gene Information

This gene encodes tumor protein p53 which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest apoptosis senescence DNA repair or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation DNA-binding and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms which can regulate p53 transcriptional activity. [provided by RefSeq Jul 2008]

Gene Symbol:   TP53
Gene Name:   tumor protein p53
Aliases:   FLJ92943, LFS1, P53, TRP53
RefSeq:   NC_000017.10 NG_017013.1 NT_010718.16
Ensembl:   ENSG00000141510
Entrez:   7157
Chromosome Mapping:   17p13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.660000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download