PrimePCR™ SYBR® Green Assay: LEP, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0031310

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: LEP, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: LEP, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0017538
Assay Design:   Intron-spanning
Chromosome Location:   7:127892170-127894561question
Amplicon Length:   121
Splice Variants Targeted:   ENST00000308868

Gene Information

This gene encodes a protein that is secreted by white adipocytes and which plays a major role in the regulation of body weight. This protein which acts through the leptin receptor functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions and is involved in the regulation of immune and inflammatory responses hematopoiesis angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq Jul 2008]

Gene Symbol:   LEP
Gene Name:   leptin
Aliases:   FLJ94114, OB, OBS
RefSeq:   NC_000007.13 NG_007450.1 NT_007933.15
Ensembl:   ENSG00000174697
Entrez:   3952
Chromosome Mapping:   7q31.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.860000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download