PrimePCR™ lncRNA SYBR® Green Assay:FLG, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qhsaLEP0211142. May amplify unspliced transcripts of an antisense gene.

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PrimePCR™ IncRNA PreAmp for SYBR® Green Assay: FLG, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ IncRNA Template for SYBR® Green Assay: FLG, Human
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   lncRNA Expression
Unique Assay ID:   qhsaLED0133902
Assay Design:   exonic
Chromosome Location:   chr1:152314028-152314129question
Amplicon Length:   72
Splice Variants Targeted:   ENST00000368799
LNCipedia Splice Variants Targeted:   lnc-FLG2-2:1

Gene Information

The protein encoded by this gene is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor profilaggrin (consisting of multiple filaggrin units of 324 aa each) which is localized in keratohyalin granules and is subsequently proteolytically processed into individual functional filaggrin molecules. Mutations in this gene are associated with ichthyosis vulgaris.[provided by RefSeq Dec 2009]

Gene Symbol:   FLG
Gene Name:   filaggrin
Aliases:   ATOD2
RefSeq:   NC_000001.10 NG_016190.1 NT_004487.19
Ensembl:   ENSG00000143631
LNCipedia:   lnc-FLG2-2
Entrez:   2312
Chromosome Mapping:   1q21.3
Gene Strand:   Not Available

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10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download