PrimePCR™ SYBR® Green Assay: Csnk1e, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qMmuCEP0055207

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Csnk1e, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Csnk1e, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0046781
Assay Design:   exonic
Chromosome Location:   15:79430072-79438778question
Amplicon Length:   71
Splice Variants Targeted:   ENSMUST00000170651 ENSMUST00000102858 ENSMUST00000080882 ENSMUST00000117786 ENSMUST00000120859 ENSMUST00000122044 ENSMUST00000144790 ENSMUST00000135519 ENSMUST00000156043 ENSMUST00000025500 ENSMUST00000004913 ENSMUST00000077968 ENSMUST00000085222 ENSMUST00000107525 ENSMUST00000107519 ENSMUST00000098090 ENSMUST00000163080 ENSMUST00000088621 ENSMUST00000037593 ENSMUST00000092892 ENSMUST00000093138 ENSMUST00000101270 ENSMUST00000090858 ENSMUST00000040915 ENSMUST00000103173 ENSMUST00000099078 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Csnk1e
Gene Name:   Casein kinase 1, epsilon
Aliases:   AI426939, AI551861, AW457082, CK1epsilon, CKIe, KC1epsilon, tau
RefSeq:   NC_000081.6 NT_039621.8
Ensembl:   ENSMUSG00000022433
Entrez:   27373
Chromosome Mapping:   15|15 E1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.950000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download