PrimePCR™ SYBR® Green Assay: Ctnnd1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qMmuCEP0055411

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Ctnnd1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Ctnnd1, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0046985
Assay Design:   exonic
Chromosome Location:   2:84601325-84601474question
Amplicon Length:   120
Splice Variants Targeted:   ENSMUST00000111696 ENSMUST00000111678 ENSMUST00000111690 ENSMUST00000111695 ENSMUST00000111677 ENSMUST00000111698 ENSMUST00000099941 ENSMUST00000111676 ENSMUST00000111694 ENSMUST00000111675 ENSMUST00000067232 ENSMUST00000111689 ENSMUST00000111687 ENSMUST00000111692 ENSMUST00000111685 ENSMUST00000111686 ENSMUST00000111688 ENSMUST00000111693 ENSMUST00000111684 ENSMUST00000111691 ENSMUST00000036811 ENSMUST00000066177 ENSMUST00000111697 ENSMUST00000111670

Gene Information

Description Not Available

Gene Symbol:   Ctnnd1
Gene Name:   Catenin (cadherin associated protein), delta 1
Aliases:   AA409437, AU019353, Catns, Ctnnd, P120, mKIAA0384
RefSeq:   NC_000068.7 NT_039207.8
Ensembl:   ENSMUSG00000034101
Entrez:   12388
Chromosome Mapping:   2 D|2 47.8 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.890000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download