PrimePCR™ SYBR® Green Assay: Aldoa, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qMmuCEP0056566

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Aldoa, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Aldoa, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0048140
Assay Design:   exonic
Chromosome Location:   7:126797343-126797461question
Amplicon Length:   89
Splice Variants Targeted:   ENSMUST00000019075 ENSMUST00000105373 ENSMUST00000049985 ENSMUST00000043149 ENSMUST00000069731 ENSMUST00000114647 ENSMUST00000071848 ENSMUST00000062108 ENSMUST00000161764 ENSMUST00000143871 ENSMUST00000173199 ENSMUST00000164578 ENSMUST00000173240 ENSMUST00000174663 ENSMUST00000173736 ENSMUST00000077930 ENSMUST00000174547 ENSMUST00000172598 ENSMUST00000127664 ENSMUST00000080123 ENSMUST00000111178 ENSMUST00000022511 ENSMUST00000034458 ENSMUST00000032934 ENSMUST00000106348 ENSMUST00000087566 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Aldoa
Gene Name:   Aldolase A, fructose-bisphosphate
Aliases:   Aldo-1, Aldo1
RefSeq:   NC_000073.6 NT_039433.8
Ensembl:   ENSMUSG00000030695
Entrez:   11674
Chromosome Mapping:   7|7 F4

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.570000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download