PrimePCR™ SYBR® Green Assay: Tyms, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: Tyms, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Tyms, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0005615
Assay Design:   Intron-spanning
Chromosome Location:   5:30061968-30063302question
Amplicon Length:   119
Splice Variants Targeted:   ENSMUST00000026846

Gene Information

This gene encodes an enzyme that catalyzes the methylation of deoxyuridylate to deoxythymidylate using 510-methylenetetrahydrofolate as a cofactor. This function maintains the thymidine-5-prime monophosphate concentration critical for DNA replication and repair. The encoded enzyme is a target for cancer chemotherapeutic agents. The majority of transcripts for this gene lack a 3' UTR (PMID: 3022294 3444407). The stop codon in these transcripts is UAA compared to the UAG found in the genome and longer transcripts as the polyA site is located within the stop codon (PMID: 3444407 2157203). A related pseudogene has been identified on chromosome 10. [provided by RefSeq Mar 2010]

Gene Symbol:   Tyms
Gene Name:   Thymidylate synthase
Aliases:   Ts
RefSeq:   NC_000071.6 NT_165760.3
Ensembl:   ENSMUSG00000025747
Entrez:   22171
Chromosome Mapping:   5 B1|5 18.2 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 34.850000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download