{"text_format":"1","workbook":"xlsx","n_sheets":3,"sheets":["Results","Input","Helper"],"not_rendered":{"charts":0,"images":1,"macros":1,"external_links":0,"defined_names":0,"comments":0,"pivot_tables":0},"unopened":[],"bounds":{"document_bytes":16777216,"sheet_cells":100000,"rows_first":2000,"rows_last":500,"rows_between":2000,"halvings":0,"cell_chars":16384,"cell_head_chars":4096},"cells_cut":0,"cells_raw":0,"read":"whole","date_system":"1900"}

{"sheet":"Results","index":0,"n_rows":492,"n_cols":12,"rows_kept":492,"cut":false,"hidden":false,"merged":[[6,0,6,4],[8,0,8,2],[2,0,2,4],[3,0,3,4],[1,0,1,4],[4,0,4,4],[5,0,5,4],[10,0,10,8],[18,0,18,11],[15,0,15,2],[15,3,15,8],[11,0,11,2],[12,0,12,2],[13,0,13,2],[14,0,14,2],[11,3,11,8],[12,3,12,8],[13,3,13,8],[14,3,14,8],[16,0,16,8]]}
[]
["Instructions "]
["1. Export data from QX software as merged wells"]
["2. Copy all data and export file from QX software to excel"]
["3. Use Green \"Insert QX data\" to select data to analyze"]
[]
[]
[]
["Workbook Version:",null,null,"1.0.0.0","RC","111df8d"]
[]
["Metrics With Explanations "]
["Median size estimate (bp)",null,null,"Median estimated fragment size in the sample based on amplified targets; given in base pairs (bp)"]
["Median estimate confidence interval (CI) ",null,null,"95% CI surrounding the median estimated fragment size"]
["Percent over 200 bp",null,null,"Percentage of the input sample with fragments ≥200 bp in size, estimated based on the ddPCR data"]
["Percent over 200 bp CI",null,null,"Confidence interval surrounding the estimation of the percent over 200 at 95%*"]
["Mass, pg / ul",null,null,"Calculated molecular weight from QX Software, based on amplified targets"]
["*The values represented by the estimates of Median Size and Percent Over 200 are based off the comparison of ddpcr data to quantitative gel based sizing methods."]
[]
["Vericheck ddPCR Residual DNA Sizing Results: HEK293"]
["Well","Sample Description 1","Sample Description 2","Sample Description 3","Sample Description 4","Median Size < 200 bp","Median Size Estimate, bp","Median CI, bp","Percent > 200 bp","Percent > 200 bp CI","pg / ul FAM Channel","pg / ul HEX Channel"]
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[{"$f":"IF(OFFSET(Helper!$A$1,(ROW(A411)*2)-1,0) = 0, \"\",  OFFSET(Helper!$A$1,(ROW(A411)*2)-1,0) )","v":""},{"$f":" IF(OFFSET(Helper!$B$1,(ROW(B411)*2)-1,0) =0, \"\", OFFSET(Helper!$B$1,(ROW(B411)*2)-1,0))","v":""},{"$f":"IF(OFFSET(Helper!$C$1,(ROW(C411)*2)-1,0)=0, \"\", OFFSET(Helper!$C$1,(ROW(C411)*2)-1,0))","v":""},{"$f":"IF(OFFSET(Helper!$D$1,(ROW(D411)*2)-1,0)=0,\"\",OFFSET(Helper!$D$1,(ROW(D411)*2)-1,0))","v":""},{"$f":"IF(OFFSET(Helper!$E$1,(ROW(E411)*2)-1,0)=0, \"\", OFFSET(Helper!$E$1,(ROW(E411)*2)-1,0))","v":""},{"$f":"IF(OR(B431=\"\",OFFSET(Helper!$T$1,(ROW(F411)*2)-1,0)=\"PosCtrl\", OFFSET(Helper!$T$1,(ROW(F411)*2)-1,0)=\"NTC\"),\"\",IF(ISNUMBER(G431),IF(G431 <200, \"Yes\", \"No\"), IF(G431 =\"<200\", \"Yes\", \"No\")))","v":""},{"$f":"IF(OR(B431=\"\",OFFSET(Helper!$T$1,(ROW(G411)*2)-1,0)=\"PosCtrl\", OFFSET(Helper!$T$1,(ROW(G411)*2)-1,0)=\"NTC\"),\"\",IF(ISNUMBER(OFFSET(Helper!$AF$1,(ROW(F411)*2)-1,0)),ROUND(OFFSET(Helper!$AF$1,(ROW(F411)*2)-1,0),0),OFFSET(Helper!$AF$1,(ROW(F411)*2)-1,0)))","v":""},{"$f":"IF(OR(B431=\"\",OFFSET(Helper!$T$1,(ROW(G411)*2)-1,0)=\"PosCtrl\", OFFSET(Helper!$T$1,(ROW(G411)*2)-1,0)=\"NTC\"),\"\",IF(OR(OFFSET(Helper!$AH$1,(ROW(G411)*2)-1,0) = \">2000\",OFFSET(Helper!$AG$1,(ROW(G411)*2)-1,0) = \"<200\"), \"LOQ\",_xlfn.CONCAT(ROUND(OFFSET(Helper!$AG$1,(ROW(G411)*2)-1,0),0),\" - \", ROUND(OFFSET(Helper!$AH$1,(ROW(G411)*2)-1,0),0))))","v":""},{"$f":"IF(OR(B431=\"\",OFFSET(Helper!$T$1,(ROW(L411)*2)-1,0)=\"PosCtrl\", OFFSET(Helper!$T$1,(ROW(L411)*2)-1,0)=\"NTC\"),\"\",IF(OFFSET(Helper!$L$1,(ROW(L411)*2)-1,0)=\"\", \"Indeterminate\",ROUND(OFFSET(Helper!$L$1,(ROW(L411)*2)-1,0)*100,2)))","v":""},{"$f":"IF(OR(B431=\"\",OFFSET(Helper!$T$1,(ROW(M411)*2)-1,0)=\"PosCtrl\", OFFSET(Helper!$T$1,(ROW(M411)*2)-1,0)=\"NTC\"),\"\",IF(OFFSET(Helper!$M$1,(ROW(M411)*2)-1,0) = \"\", \"Not Calculated\",_xlfn.CONCAT(ROUND(OFFSET(Helper!$M$1,(ROW(M411)*2)-1,0)*100,2),\" - \", ROUND(OFFSET(Helper!$N$1,(ROW(N411)*2)-1,0)*100,2))))","v":""},{"$f":"IF(OR(B431=\"\",OFFSET(Helper!$T$1,(ROW(R411)*2)-1,0)=\"PosCtrl\", OFFSET(Helper!$T$1,(ROW(R411)*2)-1,0)=\"NTC\"),\"\",IF(OFFSET(Helper!$S$1,(ROW(R411)*2)-1,0)=\"FAM\",OFFSET(Helper!$R$1,(ROW(R411)*2)-1,0),OFFSET(Helper!$R$2,(ROW(R412)*2)-1,0)))","v":""},{"$f":"IF(OR(B431=\"\",OFFSET(Helper!$T$1,(ROW(R411)*2)-1,0)=\"PosCtrl\", OFFSET(Helper!$T$1,(ROW(R411)*2)-1,0)=\"NTC\"),\"\",IF(OFFSET(Helper!$S$1,(ROW(R411)*2)-1,0)=\"FAM\",OFFSET(Helper!$R$2,(ROW(R411)*2)-1,0),OFFSET(Helper!$R$1,(ROW(R411)*2)-1,0)))","v":""}]
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