PrimePCR™ SYBR® Green Assay: LEF1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: LEF1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: LEF1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0001977
Assay Design:   Exonic
Chromosome Location:   4:109000648-109000747question
Amplicon Length:   70
Splice Variants Targeted:   ENST00000265165 ENST00000438313 ENST00000510624 ENST00000379951

Gene Information

This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq Oct 2009]

Gene Symbol:   LEF1
Gene Name:   lymphoid enhancer-binding factor 1
Aliases:   DKFZp586H0919, FLJ46390, TCF1ALPHA
RefSeq:   NC_000004.11 NT_016354.19 NG_015798.1
Ensembl:   ENSG00000138795
Entrez:   51176
Chromosome Mapping:   4q23-q25

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