PrimePCR™ SYBR® Green Assay: SULT1A4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040115

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SULT1A4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SULT1A4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037392
Assay Design:   exonic
Chromosome Location:   16:29474666-29474756question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000360423 ENST00000565290 ENST00000344620 ENST00000395400 ENST00000338971 ENST00000395138 ENST00000563322 ENST00000569485 ENST00000354723 ENST00000355544 ENST00000395137

Gene Information

Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones neurotransmitters drugs and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure (number and length of exons) is similar among family members. This gene encodes a phenol sulfotransferase with thermolabile enzyme activity. Four sulfotransferase genes are located on the p arm of chromosome 16 this gene and SULT1A3 arose from a segmental duplication. Read-through transcription exists between this gene and the upstream SLX1B (SLX1 structure-specific endonuclease subunit homolog B) gene that encodes a protein containing GIY-YIG domains. [provided by RefSeq Nov 2010]

Gene Symbol:   SULT1A4
Gene Name:   sulfotransferase family, cytosolic, 1A, phenol-preferring, member 4
Aliases:   FLJ54864, HAST3, M-PST, ST1A3/ST1A4, TL-PST
RefSeq:   NC_000016.9 NG_005304.1 NT_010393.16
Ensembl:   ENSG00000213648
Entrez:   445329
Chromosome Mapping:   -

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.540000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download