PrimePCR™ SYBR® Green Assay: CNGB1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0049557


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PrimePCR™ PreAmp for SYBR® Green Assay: CNGB1, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: CNGB1, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0042294
Assay Design:		exonic
Chromosome Location:		16:57998436-58001157question
Amplicon Length:		109
Splice Variants Targeted:		ENST00000543601 ENST00000285046 ENST00000373857 ENST00000389811 ENST00000417074 ENST00000398587 ENST00000536991 ENST00000257189 ENST00000539243 ENST00000313093 ENST00000590214 ENST00000586866 ENST00000543365 ENST00000590577 ENST00000451287 ENST00000564448 ENST00000251102 ENST00000311183 ENST00000562761

Gene Information

In humans the rod photoreceptor cGMP-gated cation channel helps regulate ion flow into the rod photoreceptor outer segment in response to light-induced alteration of the levels of intracellular cGMP. This channel consists of two subunits alpha and beta with the protein encoded by this gene representing the beta subunit. Defects in this gene are a cause of cause of retinitis pigmentosa type 45. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jan 2010]

Gene Symbol:		CNGB1
Gene Name:		cyclic nucleotide gated channel beta 1
Aliases:		CNCG2, CNCG3L, CNCG4, CNG4, CNGB1B, GAR1, GARP, RCNC2, RCNCb, RCNCbeta, RP45
RefSeq:		NC_000016.9 NT_010498.15 NG_016351.1
Ensembl:		ENSG00000070729
Entrez:		1258
Chromosome Mapping:		16q13

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999800
y-intercept	35.620000
Efficiency	101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.