PrimePCR™ SYBR® Green Assay: DYNC1I2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0049661


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PrimePCR™ PreAmp for SYBR® Green Assay: DYNC1I2, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: DYNC1I2, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0042399
Assay Design:		exonic
Chromosome Location:		2:172549402-172563877question
Amplicon Length:		71
Splice Variants Targeted:		ENST00000452242 ENST00000340296 ENST00000397119 ENST00000410079 ENST00000438879 ENST00000508530 ENST00000409197 ENST00000456808 ENST00000409317 ENST00000409773 ENST00000411953 ENST00000409453 ENST00000435234 ENST00000443458 ENST00000412370 ENST00000423910 ENST00000425485 ENST00000430778 ENST00000422646 ENST00000534253 ENST00000263811 ENST00000358002 ENST00000268712 ENST00000395849 ENST00000395857

Gene Information

Description Not Available

Gene Symbol:		DYNC1I2
Gene Name:		dynein, cytoplasmic 1, intermediate chain 2
Aliases:		DNCI2, FLJ21089, FLJ90842, IC2, MGC104199, MGC111094, MGC9324
RefSeq:		NC_000002.11 NT_005403.17
Ensembl:		ENSG00000077380
Entrez:		1781
Chromosome Mapping:		2q31.1

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.998900
y-intercept	35.380000
Efficiency	100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.