PrimePCR™ SYBR® Green Assay: OSM, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0049990


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PrimePCR™ PreAmp for SYBR® Green Assay: OSM, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: OSM, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0042728
Assay Design:		exonic
Chromosome Location:		22:30660148-30660250question
Amplicon Length:		73
Splice Variants Targeted:		ENST00000215781 ENST00000403389 ENST00000403463

Gene Information

Oncostatin M is a member of a cytokine family that includes leukemia-inhibitory factor granulocyte colony-stimulating factor and interleukin 6. This gene encodes a growth regulator which inhibits the proliferation of a number of tumor cell lines. It regulates cytokine production including IL-6 G-CSF and GM-CSF from endothelial cells. [provided by RefSeq Jul 2008]

Gene Symbol:		OSM
Gene Name:		oncostatin M
Aliases:		MGC20461
RefSeq:		NC_000022.10 NT_011520.12
Ensembl:		ENSG00000099985
Entrez:		5008
Chromosome Mapping:		22q12.2

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999200
y-intercept	35.800000
Efficiency	101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.