PrimePCR™ SYBR® Green Assay: E2F3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050691

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: E2F3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: E2F3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043429
Assay Design:   exonic
Chromosome Location:   6:20490585-20490699question
Amplicon Length:   85
Splice Variants Targeted:   ENST00000346618 ENST00000535432

Gene Information

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP) a transactivation domain enriched in acidic amino acids and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members E2F1 and E2F2 have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2011]

Gene Symbol:   E2F3
Gene Name:   E2F transcription factor 3
Aliases:   DKFZp686C18211, E2F-3, KIAA0075, MGC104598
RefSeq:   NC_000006.11 NT_007592.15
Ensembl:   ENSG00000112242
Entrez:   1871
Chromosome Mapping:   6p22

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