PrimePCR™ SYBR® Green Assay: ZAP70, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050836

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ZAP70, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ZAP70, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043574
Assay Design:   exonic
Chromosome Location:   2:98351058-98351148question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000442208 ENST00000451498 ENST00000264972 ENST00000234313

Gene Information

This gene encodes an enzyme belonging to the protein tyrosine kinase family and it plays a role in T-cell development and lymphocyte activation. This enzyme which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   ZAP70
Gene Name:   zeta-chain (TCR) associated protein kinase 70kDa
Aliases:   FLJ17670, FLJ17679, SRK, STD, TZK, ZAP-70
RefSeq:   NC_000002.11 NG_007727.1 NT_022171.15
Ensembl:   ENSG00000115085
Entrez:   7535
Chromosome Mapping:   2q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.560000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download