PrimePCR™ SYBR® Green Assay: EIF2B4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0050844


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PrimePCR™ PreAmp for SYBR® Green Assay: EIF2B4, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: EIF2B4, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0043582
Assay Design:		exonic
Chromosome Location:		2:27589786-27590063question
Amplicon Length:		111
Splice Variants Targeted:		ENST00000347454 ENST00000445933 ENST00000451130 ENST00000493344

Gene Information

Eukaryotic initiation factor 2B (EIF2B) which is necessary for protein synthesis is a GTP exchange factor composed of five different subunits. The protein encoded by this gene is the fourth or delta subunit. Defects in this gene are a cause of leukoencephalopathy with vanishing white matter (VWM) and ovarioleukodystrophy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:		EIF2B4
Gene Name:		eukaryotic translation initiation factor 2B, subunit 4 delta, 67kDa
Aliases:		DKFZp586J0119, EIF-2B, EIF2B, EIF2Bdelta
RefSeq:		NC_000002.11 NG_009305.1 NT_022184.15
Ensembl:		ENSG00000115211
Entrez:		8890
Chromosome Mapping:		2p23.3

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999600
y-intercept	35.910000
Efficiency	97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.