PrimePCR™ SYBR® Green Assay: CLU, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051146


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PrimePCR™ PreAmp for SYBR® Green Assay: CLU, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: CLU, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0043884
Assay Design:		exonic
Chromosome Location:		8:27466456-27466570question
Amplicon Length:		85
Splice Variants Targeted:		ENST00000316403 ENST00000405140 ENST00000523500 ENST00000523589 ENST00000560566 ENST00000520796 ENST00000519742 ENST00000520491 ENST00000522413 ENST00000519472 ENST00000523396 ENST00000560366 ENST00000546343

Gene Information

The protein encoded by this gene is a secreted chaperone that can under some stress conditions also be found in the cell cytosol. It has been suggested to be involved in several basic biological events such as cell death tumor progression and neurodegenerative disorders. Alternate splicing results in both coding and non-coding variants.[provided by RefSeq May 2011]

Gene Symbol:		CLU
Gene Name:		clusterin
Aliases:		APOJ, CLI, KUB1, MGC24903, SGP-2, SGP2, SP-40, TRPM-2, TRPM2
RefSeq:		NC_000008.10 NT_167187.1 NG_027845.1
Ensembl:		ENSG00000120885
Entrez:		1191
Chromosome Mapping:		8p21-p12

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999600
y-intercept	37.010000
Efficiency	93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.