PrimePCR™ SYBR® Green Assay: COPB1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051510

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: COPB1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: COPB1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044248
Assay Design:   exonic
Chromosome Location:   11:14515985-14520491question
Amplicon Length:   79
Splice Variants Targeted:   ENST00000249923 ENST00000439561 ENST00000534234 ENST00000529866 ENST00000534771 ENST00000419365

Gene Information

This gene encodes a protein subunit of the coatomer complex associated with non-clathrin coated vesicles. The coatomer complex also known as the coat protein complex 1 forms in the cytoplasm and is recruited to the Golgi by activated guanosine triphosphatases. Once at the Golgi membrane the coatomer complex may assist in the movement of protein and lipid components back to the endoplasmic reticulum. Alternatively spliced transcript variants have been described. [provided by RefSeq Jan 2009]

Gene Symbol:   COPB1
Gene Name:   coatomer protein complex, subunit beta 1
Aliases:   COPB, DKFZp761K102, FLJ10341, FLJ46444, FLJ57957
RefSeq:   NC_000011.9 NT_009237.18
Ensembl:   ENSG00000129083
Entrez:   1315
Chromosome Mapping:   11p15.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.050000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download