PrimePCR™ SYBR® Green Assay: PKLR, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0052411

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PKLR, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PKLR, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045150
Assay Design:   exonic
Chromosome Location:   1:155260216-155260359question
Amplicon Length:   114
Splice Variants Targeted:   ENST00000329021 ENST00000231721 ENST00000270233 ENST00000591949 ENST00000304613 ENST00000368571 ENST00000368572 ENST00000392414 ENST00000342741 ENST00000368539 ENST00000480071 ENST00000357296 ENST00000278060 ENST00000426431 ENST00000572740 ENST00000571194

Gene Information

The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP which is the rate-limiting step of glycolysis. Defects in this enzyme due to gene mutations or genetic variations are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   PKLR
Gene Name:   pyruvate kinase, liver and RBC
Aliases:   PK1, PKL, PKR, PKRL, RPK
RefSeq:   NC_000001.10 NG_011677.1 NT_004487.19 NW_003315906.1
Ensembl:   ENSG00000143627
Entrez:   5313
Chromosome Mapping:   1q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.810000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download