PrimePCR™ SYBR® Green Assay: CAMK2D, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0052524

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CAMK2D, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CAMK2D, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045263
Assay Design:   exonic
Chromosome Location:   4:114378565-114378662question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000394524 ENST00000513132 ENST00000511664 ENST00000342666 ENST00000515496 ENST00000514328 ENST00000394522 ENST00000505990 ENST00000379773 ENST00000508738 ENST00000454265 ENST00000429180 ENST00000418639 ENST00000394526 ENST00000296402

Gene Information

The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells the enzyme is composed of four different chains: alpha beta gamma and delta. The product of this gene is a delta chain. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Distinct isoforms of this chain have different expression patterns.[provided by RefSeq Nov 2008]

Gene Symbol:   CAMK2D
Gene Name:   calcium/calmodulin-dependent protein kinase II delta
Aliases:   CAMKD, DKFZp686G23119, DKFZp686I2288, MGC44911
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000145349
Entrez:   817
Chromosome Mapping:   4q26

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.490000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download