PrimePCR™ SYBR® Green Assay: PTGES2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0052665


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PrimePCR™ PreAmp for SYBR® Green Assay: PTGES2, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: PTGES2, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0045404
Assay Design:		exonic
Chromosome Location:		9:130885399-130886057question
Amplicon Length:		62
Splice Variants Targeted:		ENST00000338961 ENST00000449878 ENST00000277462

Gene Information

The protein encoded by this gene is a membrane-associated prostaglandin E synthase which catalyzes the conversion of prostaglandin H2 to prostaglandin E2. This protein also has been shown to activate the transcription regulated by a gamma-interferon-activated transcription element (GATE). Multiple transcript variants have been found for this gene. [provided by RefSeq Jun 2009]

Gene Symbol:		PTGES2
Gene Name:		prostaglandin E synthase 2
Aliases:		C9orf15, FLJ14038, GBF1, MGC11289, PGES2, mPGES-2
RefSeq:		NC_000009.11 NG_012488.1 NT_008470.19
Ensembl:		ENSG00000148334
Entrez:		80142
Chromosome Mapping:		9q34.11

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.993800
y-intercept	34.480000
Efficiency	108

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.