PrimePCR™ SYBR® Green Assay: TCF7L2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052686

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TCF7L2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TCF7L2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045425
Assay Design:   exonic
Chromosome Location:   10:114799878-114901071question
Amplicon Length:   107
Splice Variants Targeted:   ENST00000349937 ENST00000369397 ENST00000369395 ENST00000346198 ENST00000355995 ENST00000545257 ENST00000543371 ENST00000536810 ENST00000355717 ENST00000538897 ENST00000352065 ENST00000534894 ENST00000542695

Gene Information

This gene encodes a high mobility group (HMG) box-containing transcription factor that plays a key role in the Wnt signaling pathway. The protein has been implicated in blood glucose homeostasis. Genetic variants of this gene are associated with increased risk of type 2 diabetes. Several transcript variants encoding multiple different isoforms have been found for this gene.[provided by RefSeq Oct 2010]

Gene Symbol:   TCF7L2
Gene Name:   transcription factor 7-like 2 (T-cell specific, HMG-box)
Aliases:   TCF-4, TCF4
RefSeq:   NC_000010.10 NT_030059.13 NG_012631.1
Ensembl:   ENSG00000148737
Entrez:   6934
Chromosome Mapping:   10q25.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.160000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download