PrimePCR™ SYBR® Green Assay: TDO2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0052802


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PrimePCR™ PreAmp for SYBR® Green Assay: TDO2, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: TDO2, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0045541
Assay Design:		exonic
Chromosome Location:		4:156825188-156826233question
Amplicon Length:		73
Splice Variants Targeted:		ENST00000313949 ENST00000371075 ENST00000371100 ENST00000371085 ENST00000354359 ENST00000262935 ENST00000506072 ENST00000507590 ENST00000536354 ENST00000573403 ENST00000573644 ENST00000574434

Gene Information

This gene encodes a heme enzyme that plays a critical role in tryptophan metabolism by catalyzing the first and rate-limiting step of the kynurenine pathway. Increased activity of the encoded protein and subsequent kynurenine production may also play a role in cancer through the suppression of antitumor immune responses and single nucleotide polymorphisms in this gene may be associated with autism. [provided by RefSeq Feb 2012]

Gene Symbol:		TDO2
Gene Name:		tryptophan 2,3-dioxygenase
Aliases:		TDO, TPH2, TRPO
RefSeq:		NC_000004.11 NT_016354.19 NW_003315914.1
Ensembl:		ENSG00000151790
Entrez:		6999
Chromosome Mapping:		4q31-q32

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999100
y-intercept	35.500000
Efficiency	99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.