PrimePCR™ SYBR® Green Assay: YWHAG, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0053942


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PrimePCR™ PreAmp for SYBR® Green Assay: YWHAG, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: YWHAG, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0046681
Assay Design:		exonic
Chromosome Location:		7:75957163-75957262question
Amplicon Length:		70
Splice Variants Targeted:		ENST00000307630

Gene Information

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells and is also highly expressed in skeletal and heart muscles suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C proteins involved in various signal transduction pathways. [provided by RefSeq Jul 2008]

Gene Symbol:		YWHAG
Gene Name:		tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide
Aliases:		14-3-3GAMMA
RefSeq:		NC_000007.13 NT_007933.15
Ensembl:		ENSG00000170027
Entrez:		7532
Chromosome Mapping:		7q11.23

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999500
y-intercept	36.690000
Efficiency	93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.