PrimePCR™ SYBR® Green Assay: RCC1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0054669


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PrimePCR™ PreAmp for SYBR® Green Assay: RCC1, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: RCC1, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0047409
Assay Design:		exonic
Chromosome Location:		1:28858843-28861778question
Amplicon Length:		96
Splice Variants Targeted:		ENST00000427469 ENST00000434290 ENST00000373833 ENST00000419074 ENST00000373832 ENST00000373831 ENST00000411533 ENST00000430407 ENST00000398958 ENST00000301218 ENST00000323830 ENST00000429275

Gene Information

Description Not Available

Gene Symbol:		RCC1
Gene Name:		regulator of chromosome condensation 1
Aliases:		CHC1, RCC1-I, SNHG3-RCC1
RefSeq:		NC_000001.10 NT_004610.19
Ensembl:		ENSG00000180198
Entrez:		1104
Chromosome Mapping:		1p36.1

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999800
y-intercept	35.740000
Efficiency	98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.