PrimePCR™ SYBR® Green Assay: NPLOC4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0054789


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PrimePCR™ PreAmp for SYBR® Green Assay: NPLOC4, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: NPLOC4, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0047529
Assay Design:		exonic
Chromosome Location:		17:79532613-79534531question
Amplicon Length:		80
Splice Variants Targeted:		ENST00000331134 ENST00000573519 ENST00000571714 ENST00000572824 ENST00000374747 ENST00000539314

Gene Information

Description Not Available

Gene Symbol:		NPLOC4
Gene Name:		nuclear protein localization 4 homolog (S. cerevisiae)
Aliases:		FLJ20657, FLJ23742, KIAA1499, NPL4
RefSeq:		NC_000017.10 NT_010783.15
Ensembl:		ENSG00000182446
Entrez:		55666
Chromosome Mapping:		17qter

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999600
y-intercept	35.530000
Efficiency	98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.