PrimePCR™ SYBR® Green Assay: CSF1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0054925

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CSF1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CSF1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0047665
Assay Design:   exonic
Chromosome Location:   1:110458318-110460012question
Amplicon Length:   69
Splice Variants Targeted:   ENST00000527192 ENST00000525659 ENST00000357302 ENST00000329608 ENST00000488198 ENST00000369802 ENST00000420111 ENST00000369801 ENST00000344188

Gene Information

The protein encoded by this gene is a cytokine that controls the production differentiation and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants. [provided by RefSeq Sep 2011]

Gene Symbol:   CSF1
Gene Name:   colony stimulating factor 1 (macrophage)
Aliases:   MCSF, MGC31930
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000184371
Entrez:   1435
Chromosome Mapping:   1p21-p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.020000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download