PrimePCR™ SYBR® Green Assay: AKR1C2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0057539

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: AKR1C2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: AKR1C2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056434
Assay Design:   exonic
Chromosome Location:   10:5032067-5032158question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000380872 ENST00000434459 ENST00000380753 ENST00000421196 ENST00000407674 ENST00000380448 ENST00000263126 ENST00000578362 ENST00000582412 ENST00000580545 ENST00000578591 ENST00000580345 ENST00000579965 ENST00000583238

Gene Information

This gene encodes a member of the aldo/keto reductase superfamily which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols using NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme binds bile acid with high affinity and shows minimal 3-alpha-hydroxysteroid dehydrogenase activity. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq Dec 2011]

Gene Symbol:   AKR1C2
Gene Name:   aldo-keto reductase family 1, member C2 (dihydrodiol dehydrogenase 2; bile acid binding protein; 3-alpha hydroxysteroid dehydrogenase, type III)
Aliases:   AKR1C-pseudo, BABP, DD, DD2, DDH2, FLJ53800, HAKRD, HBAB, MCDR2
RefSeq:   NC_000010.10 NT_008705.16
Ensembl:   ENSG00000151632
Entrez:   1646
Chromosome Mapping:   10p15-p14

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