PrimePCR™ SYBR® Green Assay: SEC61G, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0058154


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PrimePCR™ PreAmp for SYBR® Green Assay: SEC61G, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: SEC61G, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0057049
Assay Design:		exonic
Chromosome Location:		7:54825222-54826876question
Amplicon Length:		62
Splice Variants Targeted:		ENST00000395535 ENST00000415949 ENST00000450622 ENST00000352861

Gene Information

The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. Oligomers of the Sec61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane. This complex consists of three membrane proteins- alpha beta and gamma. This gene encodes the gamma-subunit protein. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq Jul 2008]

Gene Symbol:		SEC61G
Gene Name:		Sec61 gamma subunit
Aliases:		SSS1
RefSeq:		NC_000007.13 NT_033968.6
Ensembl:		ENSG00000132432
Entrez:		23480
Chromosome Mapping:		7p11.2

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999700
y-intercept	35.960000
Efficiency	97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.