PrimePCR™ SYBR® Green Assay: TOP2A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0058282

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TOP2A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TOP2A, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0057177
Assay Design:   exonic
Chromosome Location:   17:38569760-38569864question
Amplicon Length:   75
Splice Variants Targeted:   ENST00000423485 ENST00000581055

Gene Information

This gene encodes a DNA topoisomerase an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation chromatid separation and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form alpha is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq Jul 2010]

Gene Symbol:   TOP2A
Gene Name:   topoisomerase (DNA) II alpha 170kDa
Aliases:   TOP2, TP2A
RefSeq:   NC_000017.10 NG_027678.1 NT_010783.15
Ensembl:   ENSG00000131747
Entrez:   7153
Chromosome Mapping:   17q21-q22

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