PrimePCR™ SYBR® Green Assay: TNC, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0058481


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PrimePCR™ PreAmp for SYBR® Green Assay: TNC, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: TNC, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0057376
Assay Design:		exonic
Chromosome Location:		9:117800548-117800659question
Amplicon Length:		82
Splice Variants Targeted:		ENST00000350763 ENST00000341037 ENST00000423613 ENST00000537320 ENST00000542877 ENST00000544972 ENST00000340094 ENST00000535648 ENST00000346706 ENST00000345230

Gene Information

This gene encodes an extracellular matrix protein with a spatially and temporally restricted tissue distribution. This protein is homohexameric with disulfide-linked subunits and contains multiple EGF-like and fibronectin type-III domains. It is implicated in guidance of migrating neurons as well as axons during development synaptic plasticity and neuronal regeneration. [provided by RefSeq Jul 2011]

Gene Symbol:		TNC
Gene Name:		tenascin C
Aliases:		150-225, GMEM, GP, HXB, JI, MGC167029, TN, TN-C
RefSeq:		NC_000009.11 NT_008470.19
Ensembl:		ENSG00000041982
Entrez:		3371
Chromosome Mapping:		9q33

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.996900
y-intercept	35.440000
Efficiency	102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.