PrimePCR™ SYBR® Green Assay: PIK3CD, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Recommended - best coverage


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PrimePCR™ PreAmp for SYBR® Green Assay: PIK3CD, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: PIK3CD, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0006002
Assay Design:		Intron-spanning
Chromosome Location:		1:9778929-9780045question
Amplicon Length:		82
Splice Variants Targeted:		ENST00000377346 ENST00000361110 ENST00000536656 ENST00000360563 ENST00000543390

Gene Information

Phosphoinositide 3-kinases (PI3Ks) phosphorylate inositol lipids and are involved in the immune response. The protein encoded by this gene is a class I PI3K found primarily in leukocytes. Like other class I PI3Ks (p110-alpha p110-beta and p110-gamma) the encoded protein binds p85 adapter proteins and GTP-bound RAS. However unlike the other class I PI3Ks this protein phosphorylates itself not p85 protein.[provided by RefSeq Jul 2010]

Gene Symbol:		PIK3CD
Gene Name:		phosphoinositide-3-kinase, catalytic, delta polypeptide
Aliases:		P110DELTA, PI3K, p110D
RefSeq:		NC_000001.10 NG_023434.1 NT_021937.19
Ensembl:		ENSG00000171608
Entrez:		5293
Chromosome Mapping:		1p36.2

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999400
y-intercept	35.290000
Efficiency	95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.