PrimePCR™ SYBR® Green Assay: BMX, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0026176


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PrimePCR™ PreAmp for SYBR® Green Assay: BMX, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: BMX, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0006458
Assay Design:		Intron-spanning
Chromosome Location:		X:15568066-15574266question
Amplicon Length:		96
Splice Variants Targeted:		ENST00000357607 ENST00000348343 ENST00000342014

Gene Information

This gene encodes a non-receptor tyrosine kinase belonging to the Tec kinase family. The protein contains a PH-like domain which mediates membrane targeting by binding to phosphatidylinositol 345-triphosphate (PIP3) and a SH2 domain that binds to tyrosine-phosphorylated proteins and functions in signal transduction. The protein is implicated in several signal transduction pathways including the Stat pathway and regulates differentiation and tumorigenicity of several types of cancer cells. Multiple alternatively spliced variants encoding the same protein have been identified.[provided by RefSeq Sep 2009]

Gene Symbol:		BMX
Gene Name:		BMX non-receptor tyrosine kinase
Aliases:		ETK, PSCTK2, PSCTK3
RefSeq:		NC_000023.10 NT_167197.1 NG_013227.1
Ensembl:		ENSG00000102010
Entrez:		660
Chromosome Mapping:		Xp22.2

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999000
y-intercept	35.140000
Efficiency	101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.