PrimePCR™ SYBR® Green Assay: POLD1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0026846


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PrimePCR™ PreAmp for SYBR® Green Assay: POLD1, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: POLD1, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0008537
Assay Design:		Intron-spanning
Chromosome Location:		19:50902695-50905161question
Amplicon Length:		144
Splice Variants Targeted:		ENST00000376930 ENST00000440232

Gene Information

This gene encodes the 125-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. Alternatively spliced transcript variants have been observed for this gene and a pseudogene of this gene is located on the long arm of chromosome 6. [provided by RefSeq Mar 2012]

Gene Symbol:		POLD1
Gene Name:		polymerase (DNA directed), delta 1, catalytic subunit 125kDa
Aliases:		CDC2, POLD
RefSeq:		NC_000019.9 NT_011109.16
Ensembl:		ENSG00000062822
Entrez:		5424
Chromosome Mapping:		19q13.3

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999600
y-intercept	33.930000
Efficiency	100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.