PrimePCR™ SYBR® Green Assay: EPHX2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0027100


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PrimePCR™ PreAmp for SYBR® Green Assay: EPHX2, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: EPHX2, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0009225
Assay Design:		Intron-spanning
Chromosome Location:		8:27373871-27379978question
Amplicon Length:		116
Splice Variants Targeted:		ENST00000521400 ENST00000517536 ENST00000521780 ENST00000380476 ENST00000518379 ENST00000521684

Gene Information

This gene encodes a member of the epoxide hydrolase family. The protein found in both the cytosol and peroxisomes binds to specific epoxides and converts them to the corresponding dihydrodiols. Mutations in this gene have been associated with familial hypercholesterolemia. Alternatively spliced transcript variants have been described. [provided by RefSeq Feb 2012]

Gene Symbol:		EPHX2
Gene Name:		epoxide hydrolase 2, cytoplasmic
Aliases:		CEH, SEH
RefSeq:		NC_000008.10 NG_012064.1 NT_167187.1
Ensembl:		ENSG00000120915
Entrez:		2053
Chromosome Mapping:		8p21

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999600
y-intercept	34.380000
Efficiency	103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.