PrimePCR™ SYBR® Green Assay: AXIN1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027462

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: AXIN1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: AXIN1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010131
Assay Design:   Intron-spanning
Chromosome Location:   16:364629-396210question
Amplicon Length:   88
Splice Variants Targeted:   ENST00000262320 ENST00000354866

Gene Information

This gene encodes a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain. The encoded protein interacts with adenomatosis polyposis coli catenin beta-1 glycogen synthase kinase 3 beta protein phosphate 2 and itself. This protein functions as a negative regulator of the wingless-type MMTV integration site family member 1 (WNT) signaling pathway and can induce apoptosis. The crystal structure of a portion of this protein alone and in a complex with other proteins has been resolved. Mutations in this gene have been associated with hepatocellular carcinoma hepatoblastomas ovarian endometriod adenocarcinomas and medullablastomas. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq Dec 2010]

Gene Symbol:   AXIN1
Gene Name:   axin 1
Aliases:   AXIN, MGC52315
RefSeq:   NC_000016.9 NG_012267.1 NT_010393.16
Ensembl:   ENSG00000103126
Entrez:   8312
Chromosome Mapping:   16p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 34.740000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download