PrimePCR™ SYBR® Green Assay: ITPA, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0027561


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PrimePCR™ PreAmp for SYBR® Green Assay: ITPA, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: ITPA, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0010383
Assay Design:		Intron-spanning
Chromosome Location:		20:3194631-3199216question
Amplicon Length:		130
Splice Variants Targeted:		ENST00000380113 ENST00000399838 ENST00000455664

Gene Information

The protein encoded by this gene hydrolyzes inosine triphosphate and deoxyinosine triphosphate to the monophosphate nucleotide and diphosphate. The encoded protein which is a member of the HAM1 NTPase protein family is found in the cytoplasm and acts as a homodimer. Defects in the encoded protein can result in inosine triphosphate pyrophosphorylase deficiency. Two transcript variants encoding two different isoforms have been found for this gene. Also at least two other transcript variants have been identified which are probably regulatory rather than protein-coding. [provided by RefSeq Jul 2008]

Gene Symbol:		ITPA
Gene Name:		inosine triphosphatase (nucleoside triphosphate pyrophosphatase)
Aliases:		C20orf37, HLC14-06-P, ITPase, dJ794I6.3
RefSeq:		NC_000020.10 NG_012093.1 NT_011387.8
Ensembl:		ENSG00000125877
Entrez:		3704
Chromosome Mapping:		20p

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999900
y-intercept	35.020000
Efficiency	100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.