PrimePCR™ SYBR® Green Assay: SP3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028290

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SP3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SP3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012072
Assay Design:   Intron-spanning
Chromosome Location:   2:174783373-174819627question
Amplicon Length:   138
Splice Variants Targeted:   ENST00000310015 ENST00000418194 ENST00000416195 ENST00000455789

Gene Information

This gene belongs to a family of Sp1 related genes that encode transcription factors that regulate transcription by binding to consensus GC- and GT-box regulatory elements in target genes. This protein contains a zinc finger DNA-binding domain and several transactivation domains and has been reported to function as a bifunctional transcription factor that either stimulates or represses the transcription of numerous genes. Transcript variants encoding different isoforms have been described for this gene and one has been reported to initiate translation from a non-AUG (AUA) start codon. Additional isoforms resulting from the use of alternate downstream translation initiation sites have also been noted. A related pseudogene has been identified on chromosome 13. [provided by RefSeq Feb 2010]

Gene Symbol:   SP3
Gene Name:   Sp3 transcription factor
Aliases:   DKFZp686O1631, SPR2
RefSeq:   NC_000002.11 NT_005403.17
Ensembl:   ENSG00000172845
Entrez:   6670
Chromosome Mapping:   2q31

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