PrimePCR™ SYBR® Green Assay: NCSTN, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028400

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: NCSTN, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NCSTN, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012336
Assay Design:   Intron-spanning
Chromosome Location:   1:160322957-160323955question
Amplicon Length:   89
Splice Variants Targeted:   ENST00000294785 ENST00000368063 ENST00000424645 ENST00000424754 ENST00000435149 ENST00000535857 ENST00000392212 ENST00000368065 ENST00000368067

Gene Information

This gene encodes a Type I transmembrane glycoprotein that is an integral component of the multimeric gamma-secretase complex. The encoded protein cleaves integral membrane proteins including Notch receptors and beta-amyloid precursor protein and may be a stabilizing cofactor required for gamma-secretase complex assembly. The cleavage of beta-amyloid precursor protein yields amyloid beta peptide the main component of the neuritic plaque and the hallmark lesion in the brains of patients with Alzheimer's disease; however the nature of the encoded protein's role in Alzheimer's disease is not known for certain. Alternatively spliced transcript variants have been described but their full-length nature has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   NCSTN
Gene Name:   nicastrin
Aliases:   APH2, KIAA0253
RefSeq:   NC_000001.10 NG_027935.1 NT_004487.19
Ensembl:   ENSG00000162736
Entrez:   23385
Chromosome Mapping:   1q22-q23

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