PrimePCR™ SYBR® Green Assay: SMC4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0029019


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PrimePCR™ PreAmp for SYBR® Green Assay: SMC4, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: SMC4, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0013742
Assay Design:		Intron-spanning
Chromosome Location:		3:160120589-160122213question
Amplicon Length:		135
Splice Variants Targeted:		ENST00000357388 ENST00000472991 ENST00000467468 ENST00000469762 ENST00000489573 ENST00000462787 ENST00000485867 ENST00000344722 ENST00000360111 ENST00000392788

Gene Information

Members of the structural maintenance of chromosomes or SMC family (e.g. SMC1A; MIM 300040) are critical for mitotic chromosome condensation in frogs and for DNA repair in mammals.[supplied by OMIM Nov 2010]

Gene Symbol:		SMC4
Gene Name:		structural maintenance of chromosomes 4
Aliases:		CAPC, SMC4L1, hCAP-C
RefSeq:		NC_000003.11 NG_022932.1 NT_005612.16
Ensembl:		ENSG00000113810
Entrez:		10051
Chromosome Mapping:		3q26.1

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999600
y-intercept	35.530000
Efficiency	97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.