PrimePCR™ SYBR® Green Assay: KPNB1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0029828

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: KPNB1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: KPNB1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0015281
Assay Design:   Intron-spanning
Chromosome Location:   17:45745661-45747085question
Amplicon Length:   128
Splice Variants Targeted:   ENST00000290158 ENST00000535458 ENST00000540627 ENST00000537679

Gene Information

Nucleocytoplasmic transport a signal- and energy-dependent process takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family. [provided by RefSeq Jul 2008]

Gene Symbol:   KPNB1
Gene Name:   karyopherin (importin) beta 1
Aliases:   IMB1, IPO1, IPOB, Impnb, MGC2155, MGC2156, MGC2157, NTF97
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000108424
Entrez:   3837
Chromosome Mapping:   17q21.32

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