PrimePCR™ SYBR® Green Assay: DDB2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0030926


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PrimePCR™ PreAmp for SYBR® Green Assay: DDB2, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: DDB2, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0016961
Assay Design:		Intron-spanning
Chromosome Location:		11:47236698-47237927question
Amplicon Length:		128
Splice Variants Targeted:		ENST00000256996 ENST00000378603 ENST00000378600 ENST00000378601

Gene Information

This gene encodes a protein that is necessary for the repair of ultraviolet light-damaged DNA. This protein is the smaller subunit of a heterodimeric protein complex that participates in nucleotide excision repair and this complex mediates the ubiquitylation of histones H3 and H4 which facilitates the cellular response to DNA damage. This subunit appears to be required for DNA binding. Mutations in this gene cause xeroderma pigmentosum complementation group E a recessive disease that is characterized by an increased sensitivity to UV light and a high predisposition for skin cancer development in some cases accompanied by neurological abnormalities. [provided by RefSeq Jul 2008]

Gene Symbol:		DDB2
Gene Name:		damage-specific DNA binding protein 2, 48kDa
Aliases:		DDBB, FLJ34321, UV-DDB2
RefSeq:		NC_000011.9 NG_009365.1 NG_023296.1 NT_009237.18
Ensembl:		ENSG00000134574
Entrez:		1643
Chromosome Mapping:		11p12-p11

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.998300
y-intercept	35.250000
Efficiency	98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.