PrimePCR™ SYBR® Green Assay: EFNB2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0031362

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: EFNB2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: EFNB2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0017612
Assay Design:   Intron-spanning
Chromosome Location:   13:107145671-107147300question
Amplicon Length:   148
Splice Variants Targeted:   ENST00000245323

Gene Information

This gene encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships ephrins are divided into the ephrin-A (EFNA) class which are anchored to the membrane by a glycosylphosphatidylinositol linkage and the ephrin-B (EFNB) class which are transmembrane proteins. This gene encodes an EFNB class ephrin which binds to the EPHB4 and EPHA3 receptors. [provided by RefSeq Jul 2008]

Gene Symbol:   EFNB2
Gene Name:   ephrin-B2
Aliases:   EPLG5, HTKL, Htk-L, LERK5, MGC126226, MGC126227, MGC126228
RefSeq:   NC_000013.10 NT_009952.14
Ensembl:   ENSG00000125266
Entrez:   1948
Chromosome Mapping:   13q33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.100000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download