PrimePCR™ SYBR® Green Assay: CD86, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: CD86, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CD86, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0021122
Assay Design:   Intron-spanning
Chromosome Location:   3:121836905-121838375question
Amplicon Length:   97
Splice Variants Targeted:   ENST00000469710 ENST00000493101 ENST00000330540 ENST00000393627 ENST00000478741 ENST00000264468

Gene Information

This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells and it is the ligand for two proteins at the cell surface of T cells CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq May 2011]

Gene Symbol:   CD86
Gene Name:   CD86 molecule
Aliases:   B7-2, B70, CD28LG2, LAB72, MGC34413
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000114013
Entrez:   942
Chromosome Mapping:   3q21

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