PrimePCR™ SYBR® Green Assay: NCAPG2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0033035


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PrimePCR™ PreAmp for SYBR® Green Assay: NCAPG2, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: NCAPG2, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0021410
Assay Design:		Intron-spanning
Chromosome Location:		7:158454994-158456929question
Amplicon Length:		90
Splice Variants Targeted:		ENST00000356309 ENST00000409423 ENST00000441982 ENST00000409339 ENST00000275830 ENST00000541468 ENST00000449727

Gene Information

Condensin complexes I and II play essential roles in mitotic chromosome assembly and segregation. Both condensins contain 2 invariant structural maintenance of chromosome (SMC) subunits SMC2 (MIM 605576) and SMC4 (MIM 605575) but they contain different sets of non-SMC subunits. NCAPG2 is 1 of 3 non-SMC subunits that define condensin II (Ono et al. 2003 [PubMed 14532007]).[supplied by OMIM Mar 2008]

Gene Symbol:		NCAPG2
Gene Name:		non-SMC condensin II complex, subunit G2
Aliases:		CAP-G2, FLJ20311, LUZP5, MTB, hCAP-G2
RefSeq:		NC_000007.13 NT_007741.14
Ensembl:		ENSG00000146918
Entrez:		54892
Chromosome Mapping:		7q36.3

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999800
y-intercept	34.760000
Efficiency	96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.