PrimePCR™ SYBR® Green Assay: E2F2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0033273

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: E2F2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: E2F2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0022067
Assay Design:   Intron-spanning
Chromosome Location:   1:23842898-23845549question
Amplicon Length:   134
Splice Variants Targeted:   ENST00000361729

Gene Information

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP) a transactivation domain enriched in acidic amino acids and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members E2F1 and E2F3 have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner and it exhibits overall 46% amino acid identity to E2F1. [provided by RefSeq Jul 2008]

Gene Symbol:   E2F2
Gene Name:   E2F transcription factor 2
Aliases:   E2F-2
RefSeq:   NC_000001.10 NT_004610.19
Ensembl:   ENSG00000007968
Entrez:   1870
Chromosome Mapping:   1p36

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